                Case: 17-10559    Date Filed: 12/06/2018   Page: 1 of 31


                                                                    [PUBLISH]



                  IN THE UNITED STATES COURT OF APPEALS

                           FOR THE ELEVENTH CIRCUIT
                             ________________________

                                   No. 17-10559
                             ________________________

                     D.C. Docket No. 8:14-cr-00496-EAK-AEP-1



UNITED STATES OF AMERICA,

                                                    Plaintiff - Appellee,

versus

ROBERT WILLIAM BARTON,

                                                    Defendant - Appellant.

                             ________________________

                     Appeal from the United States District Court
                         for the Middle District of Florida
                           ________________________

                                  (December 6, 2018)

Before MARCUS, NEWSOM and ANDERSON, Circuit Judges.

MARCUS, Circuit Judge:

         Robert Barton was charged by a grand jury sitting in the Middle District of

Florida, tried by a petit jury, and convicted in a single count of being a felon in
              Case: 17-10559     Date Filed: 12/06/2018    Page: 2 of 31


possession of a firearm in violation of 18 U.S.C. § 922(g). The evidence adduced

at trial was overwhelming, including Barton’s two confessions, first at the scene of

his arrest and later to a jailhouse informant, corroborating testimony from the

passenger in Barton’s vehicle at the time of the arrest, and deoxyribonucleic acid

(DNA) evidence directly linking Barton to the firearm. The single issue raised on

appeal is whether the district court abused its considerable discretion under

Daubert v. Merrell Dow Pharmaceuticals, Inc., 509 U.S. 579 (1993), in admitting

expert testimony concerning the DNA evidence. We can discern no error in the

trial court’s exercise of its critical gatekeeping function and, accordingly, affirm

the conviction.


                                           I.

                                          A.

      At approximately 10 p.m. on August 5th, 2014, Robert Barton was driving

with his girlfriend, Lisa Moore, and her young daughter, Emma, in Hillsborough

County, Florida. Hillsborough County Sheriff’s Department Lieutenant William

Gergel encountered the vehicle, ran its license plate, and discovered it to be a

stolen tag that did not match the vehicle. Gergel switched on his lights to pull

Barton over and called for backup. Barton quickly complied. Gergel approached

the vehicle, explained why he had pulled the car over, and asked for Barton’s

license and registration. Gergel observed that Barton appeared “nervous.” Barton

                                           2
              Case: 17-10559     Date Filed: 12/06/2018    Page: 3 of 31


produced his license and claimed he had borrowed the car from his “soon-to-be ex-

wife” and did not know that the tag was stolen. Gergel went to his car and

confirmed that the car was unregistered and that the tag and vehicle did not match.

He then asked Barton to walk to the back of the vehicle, explained that he would

probably have to impound the car because it was not registered, and asked Barton

if there was anything illegal in it. Barton initially insisted there was nothing in the

car, but when Gergel reiterated to Barton that the vehicle would be impounded and

anything inside would be discovered, Barton simply put his head down and shook

it.

      Meanwhile, one of the officers who arrived on the scene, Deputy Geraldine

Charles, saw in the back seat what appeared to be a firearm -- but turned out to be a

BB gun -- and then initiated a search of the vehicle. In the course of the search,

Deputy Charles discovered on the front floor under the passenger’s seat a loaded

.22-caliber revolver whose serial number had been scratched off. Both Barton and

Moore had felony convictions. After Deputy Charles read Barton his Miranda

rights, but before she asked him about the gun, Barton stated, “I will say the gun is

mine.” Deputy Charles told Barton that she did not want him to just “say” it was

his; she wanted Barton to tell the truth. Barton responded that the gun was in fact

his and that he had thrown it under the passenger’s seat when he saw Gergel’s




                                           3
               Case: 17-10559   Date Filed: 12/06/2018    Page: 4 of 31


lights. Barton explained that he bought the firearm from a guy off the street in

Zephyrhills.

      At trial, the Government presented substantial evidence corroborating

Barton’s confession. Moore testified that while they were being pulled over, she

felt a gun being thrown at her feet and she kicked it under the passenger’s seat.

Willie Sims, a jailhouse informant, testified that Barton had confessed while the

two shared a holding cell awaiting court appearances. Finally, the Government

introduced DNA evidence analyzed from the firearm through its expert, Candy

Zuleger, Director of Trinity DNA Solutions, a private laboratory that performed

the Barton DNA testing. She opined that the DNA profile found on the firearm

would match with only 1 out of 41 million people in the general population.

Barton presented his own DNA expert, Dr. Elizabeth Johnson, who, after extensive

examination, disagreed with Zuleger’s opinion.

                                         B.

      Before trial, Barton moved in limine to exclude the DNA evidence and

Zuleger’s testimony. The district court referred the motion to a magistrate judge,

who held a Daubert hearing at which Zuleger and Johnson both testified.

      The issues raised at the Daubert hearing and in this appeal warrant a word of

background on DNA. DNA is a complex molecule that contains the biological

coding of human traits. Within a typical human cell, DNA is wrapped tightly into


                                          4
               Case: 17-10559      Date Filed: 12/06/2018     Page: 5 of 31


forty-six chromosomes forming twenty-three pairs. Physical locations (known to

scientists as “loci”) on one chromosome correspond with physical locations on its

paired chromosome; for each locus on a chromosome that influences an attribute,

there is a related locus on its paired chromosome that also influences the attribute.

The DNA found at these loci are called “alleles,” and alleles, like chromosomes,

come in pairs. While more than 99% of DNA is identical from person to person,

scientists have determined that certain alleles are highly variable between

individuals, and have likewise determined the statistical probability of finding

those alleles in the greater population. Forensic DNA analysis focuses on these

loci and alleles known to vary widely: when a profile of such alleles from a known

person is compared to a profile of alleles from an unknown DNA sample,

statistical analysis can determine the frequency with which a sample from a

random member of the general population would also be a match.

       At the Daubert hearing, Zuleger explained Trinity’s methodology for testing

and analyzing DNA. As a laboratory accredited by the ANSI-ASQ1 National

Accreditation Board (ANAB), Trinity adheres to the International Organization for

Standardization (ISO) 17025 Standards and the FBI Quality Assurance Standards

for Forensic DNA Testing Laboratories (QAS). These standards are

operationalized through Trinity’s Forensic Biology Procedures Manual, which is

1
 ANAB is jointly named for the American National Standards Institute (ANSI) and the
American Society for Quality (ASQ).
                                             5
              Case: 17-10559     Date Filed: 12/06/2018     Page: 6 of 31


audited biennially as part of the accreditation process. The ISO standards govern

laboratory management, while the QAS standards govern DNA testing and

analysis. Trinity’s procedures manual also incorporates the guidelines set forth by

the Scientific Working Group on DNA Analysis Methods (SWGDAM), a group of

approximately fifty scientists representing federal, state, and local forensic DNA

laboratories in the United States and Canada. Zuleger testified -- and Barton does

not dispute -- that she fully adhered to Trinity’s procedures manual in this case.

      Zuleger explained that the DNA in Barton’s case was tested using the

Polymerase Chain Reaction/Short Tandem Repeat (PCR/STR) method -- a method

used by every accredited laboratory in the country. Importantly, Barton does not

challenge the reliability of PCR/STR generally. This testing process involves five

basic steps: collection, extraction, quantitation, amplification, and profile analysis.

To begin, the sample is collected from the source -- in this case, the firearm. The

DNA is then extracted from the sample and put into a solution for testing. Next,

quantitation estimates how much DNA is present in the sample. As Zuleger

testified, this is a critical step because either too much or too little DNA in the

sample can interfere with later stages of analysis: too much DNA can make the

results unreadable, while too little can prevent the lab from obtaining a DNA

profile at all. Zuleger testified, however, that even when quantitation estimates

very little or no DNA at all in the sample, the lab continues with later stages of


                                           6
              Case: 17-10559    Date Filed: 12/06/2018    Page: 7 of 31


analysis because the manufacturer’s validation studies revealed that it is

nevertheless possible to obtain usable data. Zuleger indicated that the optimal

amount of DNA is .5 nanograms, or 500 picograms, and that in this case,

quantitation estimated 210 picograms in the sample obtained from the firearm.

      Using PCR in the amplification step, the analyst then makes millions of

copies of the DNA by adding it to a “DNA kit,” a combination of chemicals that

produces the reaction. Before laboratory use, a DNA kit is subjected to validation

testing, which establishes interpretation guidelines for profile analysis. The

interpretation guidelines from validation testing on the DNA kit used in this case,

the PowerPlex Fusion, appear in Trinity’s procedures manual, and include the

parameters in which an analyst may conclude that there is a “major donor” in a

sample that contains more than one person’s DNA. Finally, Zuleger explained, the

sample is analyzed by a computer, which produces an electropherogram, a

visualization of the genetic data from which the analyst conducts profile analysis.

The alleles at each loci appear as “peaks” on the electropherogram.

      Profile analysis is relatively straightforward where the DNA in the sample

comes from one individual. One or two peaks will appear at each loci and a DNA

profile will be readily determined. The process becomes more complicated where

the DNA in the sample is a mixture from two or more individuals. In that case,

more than two peaks may appear at any given locus and an individual profile may


                                          7
              Case: 17-10559     Date Filed: 12/06/2018    Page: 8 of 31


be determined only if the analyst is able to distinguish between donors, including

by isolating a so-called “major donor.” Major donors are determined by peak-

height differences, and Trinity’s procedures manual dictates the peak-height ratios

and peak-height intensity considerations necessary to identify a major donor.

      Here, because one individual can have a maximum of two peaks at any one

locus and because there were five peaks at certain loci, Zuleger determined that the

DNA mixture came from at least three individuals. Following Trinity’s

procedures, Zuleger determined that there was a major donor in the mixture,

identified a partial DNA profile for that major donor, and then compared that

profile to the known Barton profile. During analysis, Zuleger excluded data that

fell below Trinity’s so-called “analytical threshold” and its so-called “stochastic

threshold” -- that is, data that could be subject to stochastic effects, random

distortions including allele drop-out or drop-in, which can occur particularly with

mixture samples. Zuleger testified that exclusion of this data leads to a more

conservative statistic.

      Zuleger used a modified random match probability statistical analysis, and

concluded that the frequency of occurrence of the major DNA profile derived from

the firearm sample was 1 in 41 million in the general population. The obvious

implication of Zuleger’s testimony was that it is extraordinarily unlikely that the

DNA found on the firearm came from someone other than Barton.


                                           8
              Case: 17-10559    Date Filed: 12/06/2018    Page: 9 of 31


      On the second day of the Daubert hearing, defense DNA expert Dr.

Elizabeth Johnson testified that there were three major problems with Zuleger’s

methodology and analysis: (1) that the “major donor” call was unreliable because

Trinity’s standards regarding peak-height ratios were inadequate; (2) that no

validation studies pertaining to three-or-more person mixtures had been performed

on the PowerPlex Fusion DNA kit; and (3) that the quantity of DNA material (210

picograms) was so low as to be classified as “low copy,” and thus inherently

unreliable. In support of her contentions, Johnson cited the procedures manuals

from the Harris County crime lab and the California Department of Justice, and

claimed that they are representative of what is generally accepted in the scientific

community.

      In his 16-page Report and Recommendation -- with the benefit of over six

hours of expert testimony and argument of counsel -- the magistrate judge

recommended that Barton’s motion be denied. For starters, he concluded that

Barton’s argument regarding the validation studies lacked merit because the

studies were done under well-accepted standards and Zuleger testified that the

same principles outlined in the validation studies for mixtures applied whether the

mixture contained DNA from two, three, or more donors. Meanwhile, Barton

failed to present anything demonstrating the result would have differed if Trinity’s

validation studies were conducted with complex mixtures from three or more


                                          9
             Case: 17-10559     Date Filed: 12/06/2018    Page: 10 of 31


donors. Next, the court found that while 210 picograms is below the optimal

amount of DNA for testing and may increase the risk of stochastic effects, those

effects may be “appropriately considered by trained forensic biologists under

acceptable standards.”

      The magistrate judge thus concluded that “assigning a blanket DNA

minimum threshold is misguided when the real interpretive challenge -- the

presence of stochastic effects -- is not perfectly correlated with quantity and can

typically be determined by reviewing [the] . . . test results.” And in this case,

because Zuleger “identified the presence of stochastic effects[,] she proceed[ed]

more cautiously in interpreting the data from the unknown sample, and did so by

following the interpretation guidelines memorialized from the PowerPlex Fusion

validation studies, along with Trinity’s procedures, as outlined in the Manual.”

      Finally, the magistrate judge determined that the third argument regarding

major peak heights likewise lacked merit. Because Johnson based her testimony

on the procedures utilized by two different laboratories -- both of which “went

through the same or similar accreditation process as Trinity” -- the magistrate

judge concluded that these sources offered “at best an alternative procedure in

comparison with the procedures outlined in Trinity’s Manual and the validation

studies performed by Trinity on the PowerPlex Fusion kit.” Relying on this

Court’s opinion in Quiet Technology DC-8 v. Hurel-Dubois UK Ltd., 326 F.3d


                                          10
             Case: 17-10559     Date Filed: 12/06/2018   Page: 11 of 31


1333 (11th Cir. 2003), the magistrate judge concluded that each of Barton’s three

arguments appropriately went to the weight of the evidence, and did not warrant its

exclusion under Daubert. After a de novo review, the district court adopted the

Report and Recommendation over Barton’s objections.

      On September 14, 2016, the jury returned a guilty verdict on the single count

of the indictment. The district court sentenced Barton to serve a 210-month term

of imprisonment, followed by three years of supervised release.

      On appeal, Barton argues only that the district court erred in admitting

Zuleger’s expert testimony and asks this Court to overturn his conviction and

remand the case for a new trial.

                                         II.

      “We review for abuse of discretion the district court’s decisions regarding

the admissibility of expert testimony and the reliability of an expert opinion.”

United States v. Frazier, 387 F.3d 1244, 1258 (11th Cir. 2004) (en banc); see also

Kumho Tire Co., Ltd. v. Carmichael, 526 U.S. 137, 152 (1999) (“[A] court of

appeals is to apply an abuse-of-discretion standard when it reviews a trial court’s

decision to admit or exclude expert testimony.”) (quotations and alterations

omitted).

      This abuse-of-discretion standard “recognizes the range of possible

conclusions the trial judge may reach,” Frazier, 387 F.3d at 1259, and thus affords


                                          11
             Case: 17-10559     Date Filed: 12/06/2018    Page: 12 of 31


the district court “considerable leeway” in evidentiary rulings, id. at 1258.

Accordingly, “the ‘deference that is the hallmark of abuse-of-discretion review,’

requires that we not reverse an evidentiary decision of a district court ‘unless the

ruling is manifestly erroneous.’” Id. at 1259 (first quoting Gen. Elec. Co. v. Joiner,

522 U.S. 136, 143 (1997); then quoting id. at 142). That is, this Court “must

affirm unless we find that the district court has made a clear error of judgment, or

has applied the wrong legal standard.” Id.

      Further, even an abuse of discretion will not warrant reversal where the

resulting error was harmless. We “will not overturn an evidentiary ruling and

order a new trial unless the objecting party has shown a substantial prejudicial

effect from the ruling.” Maiz v. Virani, 253 F.3d 641, 667 (11th Cir. 2001).

Substantial prejudice goes to the outcome of the trial; “where an error had no

substantial influence on the outcome, and sufficient evidence uninfected by error

supports the verdict, reversal is not warranted.” United States v. Drury, 396 F.3d

1303, 1315 (11th Cir. 2005) (quoting United States v. Hawkins, 905 F.2d 1489,

1493 (11th Cir. 1990)).

                                         III.

                                          A.

      Our analysis begins with Rule 702 of the Federal Rules of Evidence, which

provides:


                                          12
             Case: 17-10559     Date Filed: 12/06/2018    Page: 13 of 31


      A witness who is qualified as an expert by knowledge, skill, experience,
      training, or education may testify in the form of an opinion or otherwise if:
          (a) the expert’s scientific, technical, or other specialized knowledge will
              help the trier of fact to understand the evidence or to determine a fact
              in issue;
          (b) the testimony is based on sufficient facts or data;
          (c) the testimony is the product of reliable principles and methods; and
          (d) the expert has reliably applied the principles and methods to the facts
             of the case.
Fed. R. Evid. 702.

      “As the Supreme Court made abundantly clear in Daubert, Rule 702

compels the district courts to perform the critical ‘gatekeeping’ function

concerning the admissibility of expert scientific evidence.” Frazier, 387 F.3d at

1260 (quoting Daubert v. Merrell Dow Pharm., Inc., 509 U.S. 579, 589 n.7, 597

(1993)) (emphasis added in original). Because expert testimony can be both highly

persuasive and difficult for a lay jury to evaluate, the importance of this

gatekeeping function “cannot be overstated.” Id. To faithfully discharge its

gatekeeping duty, the trial court must engage in a rigorous three-part analysis and

consider whether:

      (1) the expert is qualified to testify competently regarding the matters he
      intends to address; (2) the methodology by which the expert reaches his
      conclusions is sufficiently reliable as determined by the sort of inquiry
      mandated in Daubert; and (3) the testimony assists the trier of fact, through
      the application of scientific, technical, or specialized expertise, to understand
      the evidence or to determine a fact in issue.



                                          13
               Case: 17-10559        Date Filed: 12/06/2018        Page: 14 of 31


Id. (quoting City of Tuscaloosa v. Harcros Chems., Inc., 158 F.3d 548, 562 (11th

Cir. 1998)).

       Only the second prong -- reliability -- is at issue in this case.2 “When

evaluating the reliability of scientific expert opinion, the trial judge must assess

‘whether the reasoning or methodology underlying the testimony is scientifically

valid and . . . whether that reasoning or methodology properly can be applied to the

facts in issue.’” Id. at 1261–62 (footnote omitted) (quoting Daubert, 509 U.S. at

592–93). Daubert sets forth a number of illustrative, though not exhaustive, factors

that should be considered in determining reliability, including:

       (1) whether the expert’s theory can be and has been tested; (2) whether the
       theory has been subjected to peer review and publication; (3) the known or
       potential rate of error of the particular scientific technique; and (4) whether
       the technique is generally accepted in the scientific community.

Quiet Tech. DC-8 v. Hurel-Dubois UK Ltd., 326 F.3d 1333, 1341 (11th Cir. 2003);

see also Frazier, 387 F.3d at 1262 (“These factors are illustrative, not exhaustive;

not all of them will apply in every case, and in some cases other factors will be

equally important in evaluating the reliability of proffered expert opinion.”).

       Notwithstanding its critical gatekeeping function, the trial court is just that --

a gatekeeper -- and Rule 702 is a screening procedure, not an opportunity to
2
  Indeed, the first and third prongs are beyond dispute. Zuleger has extensive scientific training
and decades of work in DNA testing laboratories, and Barton stipulated at the Daubert hearing
that she is a qualified DNA expert. As for the third prong, the presence or absence of the
Defendant’s DNA on the firearm goes to the very heart of the case -- whether the Defendant, a
convicted felon, was in fact in possession of a firearm -- and thus indisputably assists the jury “to
determine a fact at issue.” Frazier, 387 F.3d at 1260.
                                                 14
              Case: 17-10559     Date Filed: 12/06/2018     Page: 15 of 31


substitute the trial court’s judgment for that of a jury. In that regard, “it is not the

role of the district court to make ultimate conclusions as to the persuasiveness of

the proffered evidence,” Quiet Tech., 326 F.3d at 1341, and “[v]igorous cross-

examination, presentation of contrary evidence, and careful instruction on the

burden of proof are the traditional and appropriate means of attacking shaky but

admissible evidence,” id. (quoting Daubert, 509 U.S. at 596).

                                           B.

      Here, the trial court could fairly determine that Zuleger’s opinions were

based on reliable methods and sufficiently reliable application of those methods to

satisfy Daubert and warrant admission. The reliability of the basic methodology --

PCR/STR testing -- is not in dispute. Barton agrees that this type of testing is

reliable and generally accepted within the scientific community. He instead argues

that the use of this testing with the low quantity of a complex DNA mixture present

in this case, and in the absence of appropriate validation testing and interpretive

thresholds for complex mixtures, rendered the data unreliable and Zuleger’s

opinion drawn from the data inappropriate. Thus, he argues that the trial court

abused its discretion in admitting the evidence.

      We remain unpersuaded. The magistrate judge had before him the

competing scientific testimony of two qualified experts. One relied on Trinity’s

procedures manual, while the other relied on the procedures of two other


                                           15
             Case: 17-10559     Date Filed: 12/06/2018    Page: 16 of 31


laboratories. All of these procedures, though different, were reviewed and

approved by a highly regarded accrediting body. Looking at the explanations,

methodology, and procedures used by Zuleger, the magistrate judge concluded that

the Daubert standard was met. And far from “rubber stamp[ing]” the expert

testimony, as Barton claims, the magistrate judge carefully considered each of

Barton’s arguments against the reliability of Zuleger’s proposed testimony and

found the opinions reliable based on the methods she employed and in reference to

Trinity’s audited procedures. While it is true that “[t]he trial court’s gatekeeping

function requires more than simply ‘taking the expert’s word for it,’” Fed. R. Evid.

702 advisory committee’s note (2000 amends.), Zuleger explained in detail how

she formed her expert opinion based on reliable and generally accepted methods.

      Moreover, the magistrate judge credited Zuleger’s testimony only in light of

other indicia of reliability, including the auditing and accreditation process. Barton

argues for the first time in this Court that accreditation and auditing history are not

useful indicia of a lab’s adherence to reliable methodology. In support of this

argument, Barton cites a number of news articles regarding laboratories that were

either closed or reprimanded for serious lapses despite having been accredited.

This information was not presented to the trial court. But even if it had been, the

failure of accreditation and auditing to uncover problems in a few different labs




                                          16
              Case: 17-10559     Date Filed: 12/06/2018    Page: 17 of 31


does not make it an abuse of discretion for the court to have found accreditation

probative of reliability.

      Ultimately, the trial court concluded that the arguments raised by Barton did

not undermine admissibility under Daubert, but rather went to the weight of the

evidence and could be presented to the jury at trial. In so holding, the trial judge

relied heavily on our opinion in Quiet Technology, which is analogous to this case.

In that case, the appellant argued not about the reliability of the particular kind of

study or methodology, but rather that the expert “misapplied the generally valid

principles underlying” the type of study he performed. Quiet Tech., 326 F.3d at

1344. Specifically, appellant argued that the expert had failed to use the proper

equations in calculating certain data that was ultimately put into analysis software.

Similarly here, Barton argues that Zuleger failed to perform certain calculations

that would have altered the data ultimately used to determine that the frequency of

occurrence of the major DNA profile derived from the firearm sample was 1 in 41

million in the general population. The Quiet Technology Court found that the

“identification of such flaws in generally reliable scientific evidence is precisely

the role of cross-examination.” Id. at 1345; see also Rosenfeld v. Oceania Cruises,

Inc., 654 F.3d 1190, 1193 (11th Cir. 2011) (“Indeed, ‘in most cases, objections to

the inadequacies of a study are more appropriately considered an objection going

to the weight of the evidence rather than its admissibility.’”) (quoting Hemmings v.


                                           17
             Case: 17-10559      Date Filed: 12/06/2018    Page: 18 of 31


Tidyman’s Inc., 285 F.3d 1174, 1188 (9th Cir. 2002)). The Court concluded that

“[b]ecause [the expert’s] methods and results were discernible and rooted in real

science -- i.e., were ‘intellectual[ly] rigor[ous]’ -- they were empirically testable.

As such, they were subject to effective cross examination and, indeed, were

questioned vigorously” by the appellant at trial. Quiet Tech., 326 F.3d at 1346

(quoting Kumho Tire, 526 U.S. at 152). The same is true here: Zuleger testified at

trial, Barton vigorously cross-examined Zuleger and offered the rebutting

testimony of Johnson, another expert, and the jury was left to evaluate the

evidence. Moreover, the district judge appropriately instructed the jury that they

were to treat expert scientific testimony like “any other witness’s testimony” and

“decide for [themselves] whether to rely upon the opinion.”

      The trial court rightly reached its decision based on an evaluation of the

foundations of Zuleger’s testimony and the failure of the defense to rebut it with

anything but the testimony of a competing expert, who employed the same general

methodology. Indeed, it would have gone beyond the gatekeeping function of the

trial court to exclude Zuleger’s testimony on the basis of a credibility

determination favoring Johnson’s. See Ambrosini v. Labarraque, 101 F.3d 129,

141 (D.C. Cir. 1996) (“The district court[] err[ed] . . . [by] misconce[iving] of the

limited ‘gatekeeper’ role envisioned in Daubert. By attempting to evaluate the

credibility of opposing experts and the persuasiveness of competing scientific


                                           18
             Case: 17-10559      Date Filed: 12/06/2018   Page: 19 of 31


studies, the district court conflated the questions of the admissibility of expert

testimony and the weight appropriately to be accorded such testimony by a fact

finder.”) (cited approvingly in Quiet Tech., 326 F.3d at 1341). The issues raised

by Johnson’s competing testimony went to the weight owed Zuleger’s expert

opinion, and were properly left to the jury. Under these circumstances, the trial

court did not abuse its discretion in allowing the presentation of Zuleger’s expert

testimony to the jury.

                                          IV.

      On appeal, Barton offers substantial additional evidence on the reliability of

Zuleger’s methodology, including several scientific journal articles and the

updated 2017 SWGDAM guidelines. Much of this evidence is potentially

probative of reliability. The 2017 SWGDAM guidelines in particular contain an

important update relating to validation studies for greater-than-two-person

mixtures. Barton acknowledges on appeal that Trinity’s procedures were

developed in accordance with the 2010 SWGDAM guidelines, but argues that

those guidelines were primarily designed to cover single-source samples and two-

person mixtures. He points to SWGDAM’s Frequently Asked Questions (FAQs)

relating to the 2010 guidelines, which note that the “nuances and limitations to the

interpretation of . . . more complex mixtures . . . are not fully explored in the 2010

guidelines,” and thus “encourage” laboratories “to perform additional validation


                                          19
              Case: 17-10559     Date Filed: 12/06/2018    Page: 20 of 31


studies of complex mixtures to further their understanding of the issues related to

these challenging samples.”

      The 2017 guidelines, on the other hand, explicitly address such complex

mixtures, and take a strong position on mixture validation testing. Under the new

guidelines:

      Interpretation guidelines for mixtures must be based on mixture studies
      conducted using known contributors that represent the number of
      contributors and the range of general mixture types (e.g., mixture
      proportions and template quantities) for which the procedure will be used in
      casework. If a laboratory will be interpreting mixtures containing stochastic
      level data, the validation studies on which the interpretation guidelines are
      based should contain mixtures with stochastic level data.

SWGDAM Interpretation Guidelines for Autosomal STR Typing by Forensic

DNA Testing Laboratories, § 2.3 (2017) (emphasis added). In other words, the

updated SWGDAM guidelines support Barton’s claim that analysis of a low-

quantity three-person mixture should be based on interpretation guidelines drawn

from validation studies performed on low-quantity three-person mixtures.

Validation studies go to the heart of reliability. See Daubert, 509 U.S. at 590

(“Proposed testimony must be supported by appropriate validation -- i.e., ‘good

grounds,’ based on what is known.”). Accordingly, the 2017 guidelines modifying

scientific standards for validation studies are potentially important evidence cutting

against reliability. Nevertheless, these 2017 SWGDAM guidelines were not

available at the time of the trial and thus were not presented to the district court.


                                           20
              Case: 17-10559     Date Filed: 12/06/2018     Page: 21 of 31


The important question before us, then, is whether and how to consider these new

guidelines -- or the scientific journal articles -- in the context of an appeal.

      The Sixth Circuit confronted a strikingly similar question in United States v.

Bonds, 12 F.3d 540 (6th Cir. 1993). There, appellant asked the court to take

judicial notice of a report of the National Research Committee of the National

Academy of Sciences, issued a year after appellant’s conviction, which questioned

the reliability of certain FBI DNA interpretation procedures. Id. at 552. First, the

court noted that the Federal Rules of Appellate Procedure confine the record on

appeal to the original papers and exhibits filed in the district court, the transcript of

proceedings, if any, and a certified copy of the docket entries prepared by the

district clerk. Id. (citing Fed. R. App. P. 10(a)). Thus, the report, which was not

before the district court, could not be considered on appeal. The court likewise

declined to take judicial notice of the report because it did not contain the sort of

facts “not subject to reasonable dispute” contemplated by the Federal Rule’s

judicial notice provision. Id. at 553. Most importantly, the Sixth Circuit reasoned

that consideration of the report would subvert the fact-finding role of the district

court and inappropriately impose de novo review on appeal. Id. (“Most

importantly, if we were to look at new scientific data available to us but not

available to the district court that made the admissibility determination, we would

not be confining ourselves to reviewing the district court’s admissibility ruling, but


                                           21
                 Case: 17-10559   Date Filed: 12/06/2018   Page: 22 of 31


would be making a de novo determination based on post-conviction developments

or articles.”)

       We agree with the Sixth Circuit and reach the same result here. The 2017

SWGDAM guidelines are not and, given their timing, could not be part of the

record on appeal. We thus decline to consider them. The same is true for the

medical journal articles Barton newly submits. See United States v. Diaz, 630 F.3d

1314, 1334 n.13 (11th Cir. 2011) (“In his brief, Diaz has citations to medical

journal articles that he did not submit as evidence to the district court. This

evidence is not part of the record on appeal, and we decline to consider it.”) (citing

Bonds, 12 F.3d at 552).

       Barton now argues that this Court should consider the additional evidence

anyway, primarily because it was the Government’s burden, not his, to lay a proper

foundation for its proffered expert opinion testimony. This argument misses the

mark. The Supreme Court has made it abundantly clear that the question for an

appellate court is only whether the trial court abused its discretion in making its

gatekeeping determination. See Joiner, 522 U.S. at 146 (“We hold, therefore, that

abuse of discretion is the proper standard by which to review a district court’s

decision to admit or exclude scientific evidence.”). We cannot find an abuse of

discretion based on our consideration of new evidence -- evidence never

considered by the trial court and never explained by the DNA expert -- on appeal.


                                           22
             Case: 17-10559     Date Filed: 12/06/2018    Page: 23 of 31


The trial court simply could not have abused its discretion in failing to credit

information not presented in that forum because the evidence did not yet exist.

Based on the expert evidence presented to the trial court, we cannot say that it

abused its discretion.

      Indeed, to take the opposite position would effectively mandate de novo

factual review in this Court; we would be compelled to consider all the evidence

afresh in light of the new evidence rather than focus on what was before the trial

court. This would utterly undermine the deference we owe to the district court and

profoundly alter the role of an appellate court. The virtues of deference to trial

courts in evidentiary determinations are many, and the sound policy underpinnings

for our deferential standard of review illustrate the danger of ignoring it. Chief

among those underpinnings is that district courts are simply best-positioned to

make fact-intensive evidentiary calls. See Salve Regina Coll. v. Russell, 499 U.S.

225, 233 (1991) (“Those circumstances in which Congress or [the] Court has

articulated a standard of deference for appellate review of district-court

determinations reflect an accommodation of the respective institutional advantages

of trial and appellate courts.”); United States v. Brown, 415 F.3d 1257, 1265 (11th

Cir. 2005). It is undeniable that Daubert and its progeny set forth an

individualized, fact-laden reliability inquiry. Daubert, 509 U.S. at 593 (“Many

factors will bear on the inquiry, and we do not presume to set out a definitive


                                          23
              Case: 17-10559     Date Filed: 12/06/2018      Page: 24 of 31


checklist or test.”); see also Kumho Tire, 526 U.S. at 150 (explaining that

Daubert’s “gatekeeping inquiry must be ‘tied to the facts’ of a particular ‘case’”).

Daubert’s suggested factors that a district court might employ include whether the

scientific theory can be and has been tested and the known and potential error rate

of a particular technique. Daubert, 509 U.S. at 593–94. These are potentially

complex factual questions. And if one principle is clear, it is that appellate courts

are not fact finders. The institutional advantages of trial courts in such an inquiry -

- and thus the virtues of deferential review by appellate courts -- are numerous.

For starters, “[t]he trial judge’s major role is the determination of fact, and with

experience in fulfilling that role comes expertise.” Anderson v. City of Bessemer

City, 470 U.S. 564, 574 (1985) (explaining the underpinnings of clear error review

of factual findings). We defer, in part, therefore, because district judges (and

juries) are the expert fact finders of the federal courts.

      Additionally, the trial court has before it the evidence and expert witnesses

themselves. They are subject to confrontation and cross-examination in that court.

Because it has “first-hand access to documentary evidence and is physically

proximate to testifying witnesses,” Brown, 415 F.3d at 1265 (quoting United States

v. Jernigan, 341 F.3d 1273, 1285 (11th Cir. 2003)), the district court can evaluate

the witnesses and, where necessary, clarify its understanding of the complex

science and its application to the case. See Jernigan, 341 F.3d at 1285 (noting in


                                           24
              Case: 17-10559      Date Filed: 12/06/2018     Page: 25 of 31


the Rule 403 context that “the district court is uniquely situated to make nuanced

judgments on questions that require careful balancing of fact-specific concepts like

probativeness and prejudice”). Any effort at duplicating at the appellate level the

trial court’s familiarity with the record or nuanced insights gained throughout the

life of the case is wholly impractical, if not impossible. Id.

       Moreover, where the law affords discretion, deference not only respects the

choice and the judgment inherent in discretion, but also promotes certainty for the

litigants. Almost nowhere is the rationale for abuse-of-discretion review more

powerful than when the district court makes an evidentiary ruling. Evidentiary

issues -- whether particular evidence is relevant, or cumulative, or unfairly

prejudicial -- frequently demand the trial court make close judgment calls. There

may be no clearly correct answer and, indeed, reasonable minds can readily

disagree on the appropriateness of admitting or excluding particular evidence. Any

rule allowing appellate courts to supplant their own judgment on these close

evidentiary calls would create enormous uncertainty -- uncertainty that would

come with little benefit for the development of the law. Quite simply, de novo

appellate review of “questions involving ‘multifarious, fleeting, special, narrow

facts that utterly resist generalization,’ . . . is unlikely to establish clear guidelines

for lower courts.” Cooter & Gell v. Hartmarx Corp., 496 U.S. 384, 404–05 (1990)




                                            25
             Case: 17-10559     Date Filed: 12/06/2018    Page: 26 of 31


(quoting Pierce v. Underwood, 487 U.S. 552, 561–62 (1988)) (describing the fact-

specific inquiry in a Rule 11 sanctions determination).

      This is not to say that a defendant is left without any remedy if

postconviction scientific advances cast doubt on the reliability of forensic evidence

used to establish his guilt. As science progresses, and “[s]cientific conclusions are

subject to perpetual revision,” Daubert, 509 U.S. at 597, and best methods are

replaced by those still better, reliability is an inherently moving target. Id. While

methods of forensic analysis, once widely accepted, can be discredited or discarded

altogether, the law has mechanisms to revisit convictions arguably infected by

unreliable evidence. Here, for instance, Barton could seek a new trial. Under Rule

33 of the Federal Rules of Criminal Procedure, a district court may grant a motion

for a new trial “if the interest of justice so requires.” Fed. R. Crim. P. 33(a). A

motion for a new trial based on “newly discovered evidence” must be filed within

three years after the guilty verdict. Fed. R. Crim. P. 33(b)(1).

      We acknowledge that a Rule 33 motion is an uphill climb. Such motions

“are highly disfavored in the Eleventh Circuit and should be granted only with

great caution.” United States v. Campa, 459 F.3d 1121, 1151 (11th Cir. 2006). To

succeed on a Rule 33 motion based on newly discovered evidence, a defendant

must demonstrate the following conditions:

      (1) the evidence must be discovered following trial; (2) the movant must
      show due diligence to discover the evidence; (3) the evidence must not be
                                          26
              Case: 17-10559      Date Filed: 12/06/2018    Page: 27 of 31


      merely cumulative or impeaching; (4) the evidence must be material to
      issues before the court; and (5) the evidence must be of such a nature that a
      new trial would probably produce a new result.

United States v. DiBernardo, 880 F.2d 1216, 1224 (11th Cir. 1989). Given the

law’s interest in certainty and finality, that the new trial standard is difficult to

meet is a feature, not a flaw; only under limited circumstances should the law

disturb the considered judgments of trial courts and juries after the fact.

Nevertheless, real remedies exist to correct injustices where postconviction

scientific developments seriously undermine the validity of those judgments. But

turning appellate courts into basic fact finders is not one of them.

                                            V.

      Beyond all of that, any error in admitting the DNA evidence here -- and we

see none -- was harmless. The Federal Rules of Criminal Procedure direct courts

to disregard “[a]ny error, defect, irregularity, or variance that does not affect

substantial rights.” Fed. R. Crim. P. 52(a). At the appellate level, we read this rule

in conjunction with the federal harmless-error statute, which compels us to decide

appeals “without regard to errors or defects which do not affect the substantial

rights of the parties.” 28 U.S.C. § 2111. “In applying this test, we use the

Kotteakos standard, which teaches that a nonconstitutional error requires reversal

only if it resulted ‘in actual prejudice because it had substantial and injurious effect

or influence in determining the jury’s verdict.’” United States v. Guzman, 167


                                            27
              Case: 17-10559     Date Filed: 12/06/2018     Page: 28 of 31


F.3d 1350, 1353 (11th Cir. 1999) (quoting United States v. Lane, 474 U.S. 438,

449 (1986); Kotteakos v. United States, 328 U.S. 750, 776 (1946)); see also

Frazier, 387 F.3d at 1266 n.20. In other words, “where an error had no substantial

influence on the outcome, and sufficient evidence uninfected by error supports the

verdict, reversal is not warranted.’” Drury, 396 F.3d at 1315 (quoting Hawkins,

905 F.2d at 1493); Peat, Inc. v. Vanguard Research, Inc., 378 F.3d 1154, 1162 (11th

Cir. 2004) (“Our cases, consistent with Rule 61 of the Federal Rules of Civil

Procedure, hold that a new trial is warranted only where the error has caused

substantial prejudice to the affected party (or, stated somewhat differently, affected

the party’s ‘substantial rights’ or resulted in ‘substantial injustice’). Notwithstanding

the difference in terminology, the inquiry is always the same -- how much of an

effect did the improperly admitted or excluded evidence have on the verdict?”).

      Though the harmlessness inquiry is necessarily case-specific, our precedents

set forth a number of useful factors in answering the question, including “the

number of errors, the closeness of the factual disputes (i.e., the strength of the

evidence on the issues affected by the error), . . . the prejudicial effect of the

evidence at issue[,] . . . whether counsel focused on the evidence during the trial,

and whether any cautionary or limiting instructions were given.” Id. at 1162.

      We are mindful both that DNA evidence is powerful and it could be highly

prejudicial. Nevertheless, the other evidence of Barton’s guilt was overwhelming.


                                           28
             Case: 17-10559     Date Filed: 12/06/2018   Page: 29 of 31


For starters, Barton was driving the car in which the gun was found. Barton

confessed not once, but twice. At the scene of his arrest, Barton told Deputy

Charles that the gun was his and that he had thrown it under the passenger’s seat

when he saw Gergel’s lights. He said he got it from a guy off the street in

Zephyrhills. While sharing a holding cell at the courthouse with Sims, Barton

again confessed. When Barton discovered that Sims was from Pasco County,

Barton began naming possible common acquaintances from the area. When it

turned out they both knew Rosa Linda from Lock Street in Dade City (a

neighboring town of Zephyrhills), Barton told Sims he purchased his firearms from

Rosa Linda, including the .22-caliber revolver with a scratched-off serial number

for which he was then facing charges. Sims said Barton also told him that Barton

was driving his wife’s car with another woman when he was pulled over, and that

he attempted to wipe fingerprints off the gun because he knew with his record that

the police were likely to search the car. Sims’s detailed knowledge of the crime

and arrest -- though not entirely consistent with Barton’s initial confession -- made

his testimony particularly credible.

      Barton’s confessions were corroborated in still other ways. Both at the

scene and later at trial, his passenger, Lisa Moore, implicated him, and her story

matched with his. Moore said that after seeing Sergeant Gergel’s lights, she felt a

gun being thrown at her feet, and that she kicked it under the passenger’s seat,


                                         29
              Case: 17-10559      Date Filed: 12/06/2018     Page: 30 of 31


precisely where the gun was found. Moore gave a written statement to police on

the night of the arrest that similarly said Barton had thrown the gun at her feet. All

told, this evidence painted a powerful picture of Barton’s guilt. Thus, it is clear

that sufficient evidence uninfected by any conceivable error in admitting the DNA

testimony supports Barton’s conviction. Drury, 396 F.3d at 1315.

       In addition to the strength of the other evidence against Barton, the trial

judge properly instructed the jury on weighing expert testimony. As we have

repeatedly found, proper instruction to the jury on weighing expert testimony may

render errors in admitting the evidence harmless. See United States v. Myers, 972

F.2d 1566, 1578 (11th Cir. 1992) (“This error, however, was harmless in light of

the court’s instructions to the jury that it was their ‘duty to decide . . . the specific

facts,’ and whether to ‘accept . . . [and] rely upon an expert witness.’”); United

States v. Herring, 955 F.2d 703, 708–09 (11th Cir. 1992) (finding any possible

error harmless where the district court “carefully instructed the jury” and

“admonished [it] to give no special deference to the expert testimony”). Here,

using this Circuit’s pattern jury instruction, the district judge told the jurors to treat

expert scientific testimony like “any other witness’s testimony” and “decide for

[themselves] whether to rely upon the opinion.” After hearing the extensive

testimony from dueling DNA experts, the jury was properly directed to evaluate




                                            30
                Case: 17-10559      Date Filed: 12/06/2018   Page: 31 of 31


the competing testimony of Zuleger and Johnson and decide for itself what weight,

if any, to accord the DNA evidence.

          In short, even if we were to conclude that the district court had abused its

discretion in allowing the expert testimony of Ms. Zuleger, any claimed error

would have been harmless. The evidence was overwhelming and we can find no

reasonable likelihood that the claimed error affected the Defendant’s substantial

rights.

          Accordingly, we affirm.

          AFFIRMED.




                                             31
